Dynamic metabolic imaging with 3D spiral CSI for hyperpolarized [2-13C]pyruvate
Date
Sonal Josan, Ph.D.
Senior Research Scientist
Department of Radiology
Stanford University
Hosted by Peder Larson and the Hyperpolarized MRI Technology Resource Center
Abstract: Spectroscopic imaging with hyperpolarized 13C substrates has been widely used to measure metabolic processes in real time in vivo. The most widely used substrate, [1-13C]pyruvate, allows the assessment of pyruvate dehydrogenase (PDH) flux, which converts pyruvate to acetyl-CoA releasing the 13C label as 13CO2/13C-bicarbonate. There has also been growing interest in using hyperpolarized [2-13C]pyruvate, to follow the 13C label into downstream metabolic products, indicating incorporation of acetyl-CoA into the tricarboxylic acid (TCA) cycle and other metabolic pathways. Chemical shift imaging (CSI) with [2-13C]pyruvate is challenging given the wide spectral dispersion of the resonances, e.g. over 5000 Hz at 3T between C2 resonances of pyruvate and alanine. This talk presents 3D spiral CSI with spectral undersampling for metabolic imaging of hyperpolarized [2-13C]pyruvate demonstrating results from application to brain, heart and liver.
America/Los_Angeles publicType
Time Duration
Sonal Josan, Ph.D.
Senior Research Scientist
Department of Radiology
Stanford University
Hosted by Peder Larson and the Hyperpolarized MRI Technology Resource Center
Abstract: Spectroscopic imaging with hyperpolarized 13C substrates has been widely used to measure metabolic processes in real time in vivo. The most widely used substrate, [1-13C]pyruvate, allows the assessment of pyruvate dehydrogenase (PDH) flux, which converts pyruvate to acetyl-CoA releasing the 13C label as 13CO2/13C-bicarbonate. There has also been growing interest in using hyperpolarized [2-13C]pyruvate, to follow the 13C label into downstream metabolic products, indicating incorporation of acetyl-CoA into the tricarboxylic acid (TCA) cycle and other metabolic pathways. Chemical shift imaging (CSI) with [2-13C]pyruvate is challenging given the wide spectral dispersion of the resonances, e.g. over 5000 Hz at 3T between C2 resonances of pyruvate and alanine. This talk presents 3D spiral CSI with spectral undersampling for metabolic imaging of hyperpolarized [2-13C]pyruvate demonstrating results from application to brain, heart and liver.