Equipment and Facilities

Our Center is headquartered at Mission Bay in Byers Hall.  The Surbeck Laboratory for Advanced Imaging and The Biomedical NMR Laboratory conducts Research and Development with state-of-the-art instruments and technology.

 

Hyperpolarized MRI Technology Center Equipment

Commercial Oxford Instrument HyperSense

 
• Magnet field strength: 3.35T actively shielded
• Polarized sample temperature: ≥ 1.4K
• Polarization transfer source: 94GHz (up to 200mW)
• Polarization sample volume: up to 200ul, typical < 50ul
• Polarization sample quantity: 1 sample cup/ dissolution
• Polarization agent: Trityl radical, typical
• Dissolution volume: 3 to 10ml, typically water based
• Helium consumption / dissolution < 2L, typically 1L / sample
• Helium refill volume: 65L from min. level
• Nitrogen refill volume: 60L from min. level
• Sample polarization time: typically 15 mins to 2 hours
• Sample dissolution time: > 10 secs
• Sample 13C S/N enhancement: up to 10,000 fold

 

HyperSense DNP Polarizer

• Field strength: 3.35T
• Microwave source: Integrated 94GHz with 0.5Hz sweep, (user selectable) up to 100mW
• Sample volume: 10-200ul
• Dissolution volume: 3.5-5ml
• Dissolution solvent: water
• Temperature: (selectable) 1.3°K
• Nuclei: 13C, 15N, 31P, 29Si and other spin ½ nuclei
• Polarization duration: 15min – 6hrs
• Polarization agent: 2-5mg ‘Trityl radical’ per sample
• Helium consumption: 2L per dissolution

 

 

Proof-of-Concept Sterile Polarizer

• Magnet field strength: 5T shielded
• Polarized sample temperature: ≥ 1.2K
• Polarization transfer source: 140GHz (up to 50mW)
• Polarization sample volume: up to 2ml
• Polarization sample quantity: 1 sample cup/dissolution
• Polarization agent: Trityl radical, typical
• Dissolution volume: 15 to 65ml, typically water based
• Helium consumption/dissolution: < 3L, typically 2L/sample
• Helium refill volume: 65L from min. level
• Nitrogen refill volume: 60L from min. level
• Sample polarization time: typically 30 mins to 2 hours
• Sample dissolution time: > 30 secs
• Sample 13C S/N enhancement: up to 10,000 fold1
• Dissolved sample volume: up to 55ml
• Dissolved sample temperature: controlled, typical 37°C
• Polarization agent filtration: C18 column
• QC parameter measurement: pH, temperature, % polarization, weight

 

Oxford Instruments Test-bed Polarizer

• Magnet field strength: 3.35T actively shielded
• Nuclei: 13C, 15N, 29SI
• Polarized sample temperature: ≥ 1.4K
• Polarization transfer source: 94GHz (up to 200mW)
• Polarization sample volume: up to 200ul, typical < 50ul
• Polarization sample quantity: 1 sample cup/ dissolution
• Polarization agent: Trityl radical, typical
• Dissolution volume: 3 to 10ml, typically water based
• Helium consumption / dissolution < 2L, typically 1L / sample
• Helium refill volume: 65L from min. level
• Nitrogen refill volume: 60L from min. level
• Sample polarization time: typically 15 mins to 2 hours
• Sample dissolution time: > 10 secs
• 13C S/N enhancement: up to 10,000 fold1

 

3.35T GE SpinLab Polarizer

• Polarized sample temperature: ≥ 0.8K
• Polarization transfer source: 94GHz (up to 100mW)
• Polarization sample volume: 60ul to 1.5ml
• Polarization sample quantity: up to 4 sample vials simultaneous
• Daily sample quantity: up to 12 samples/regeneration cycle
• Polarization agent: Trityl radical, typical
• Dissolution volume: 15ml to 50ml, typically water based
• Helium consumption/dissolution: N.A.
• Sample polarization time: typically 15 mins to 2.5 hours
• Sample dissolution time: > 10 secs
• Sample 13C S/N enhancement: up to 10,000 fold1

 

5T GE SpinLab Multisample Polarizer and QC Unit Prototype

• Magnet field strength: 5T actively shielded
• Polarization transfer source: 140GHz (up to 50mW)
• Polarization sample volume: 60ul to 1.5ml
• Polarization sample quantity: up to 4 sample vials simultaneous
• Daily sample quantity: up to 12 samples/ regeneration cycle
• Polarization agent: Trityl radical, typical
• Dissolution volume: 15 to 65ml, typically water based
• Helium consumption/dissolution: N.A.
• Helium recycling/regeneration: Cryo pump cooled
• Sample polarization time: typically 30 mins to 3 hours
• Sample dissolution time: > 20 secs
• Sample 13C S/N enhancement: up to 10,000 fold1
• Dissolved sample volume: up to 55ml
• Dissolved sample temperature: controlled, typical 37°C
• Polarization agent filtration: simple porous filtering

 

Labconco Purifier 4ft Horizontal Clean Bench and Getinge Isolator  

Labconco Purifier 4ft
•SO Class 5 work station – particulate free environment
• Feature double-gasket, negative pressure leak protection
• 99.99% efficient HEPA, front-mounted light and blower switches
 
Getinge Isolator
• 4 Glove Isolator Laminar Airflow 
• 190 DPTE alpha flange for sterile filtrated of fluids (WFI, buffer, helium) 
• Positive pressure control – ventilation system made of 2 blowers, one in inlet and one in outlet
• Siemens PC control
• Lift-up front window
• Custom designed rack and shelves for process articles bio-decon
• Glove extender 
• Enclosure is equipped with one temperature/humidity probe. Probe is able to measure the humidity level for the bio-decontamination cycles 
• Bio-decontaminated using the integrated sterilizer Steritrace® II.  Sterilizer works with the chemical agent H2O2

7T GE 950 Whole Body MR Scanner

• Signa MR950 7.0T
• 900mm superconducting magnet with passive room shield
• XRMb gradient coil delivering 50 mT/m at 200 T/m-s
• 61cm bore
• Very high EPI stability – typically less than 0.1% detrended RMS fluctuation in phantom over 10 minutes
• Dual phase offset transmitter channels – 8 kW total input power for 1H, or 4kW 1H/4 kW MNS
• 32 receive channels for 1H; up to 8 receive channels for MNS
• Nova 2 channel transmit/32 channel receive head coil

 

3T GE 750 Whole Body MR Scanner

• Discovery MR750 3.0T
• 18 superconducting High Order Shims, including X, Y, Z, XY, XZ, YZ Z2, X2-Y2
• Active shielding technology
• 60cm bore
• Optical RF technology with 165dB dynamic range
• Optics allow for scalability up to 136 coil connections
• Technology enables multi-channel broadband receive for multi-nuclear studies
• Vector Reconstruction Engine (VRE)
• Delivers processing power to reconstruct 32-channels of high-resolution 3D volumetric data
• Water cooled power electronics
• Extreme Gradient Driver (XGD)
• Power and duty cycle to support 50mT/m peak amplitude at a slew-rate of 200mT/m/s on all 3-axis simultaneously
• Continuous peak gradient performance with 100% duty cycle
• RF amplifier: for body coil, water cooled, solid-state 35 kW RF amplifier. In addition amplifier supports a 100W continuous-wave output mode for proton-decoupling and spin-labeling research.
• Multichannel spectroscopy with 8-kW broadband amplifier and 8 channel receiver converter. Carbon and Phosphorous TR switches.

 

500 MHz HR-MAS Spectrometer

• Varian UNITY/NOVA 11.7T (500MHz 1H) Spectrometer
• Narrow bore
• Equipped with gHx indirect and direct detection nanoprobes
• H-X pulsed field gradient indirect detection 5mm and 10mm liquids probe
• Capable of 1H and hetero-or multi-nuclear detection (31P, 13C, 15N)
• Equipped with a variable temperature control device

 

 

600 MHz Wide Bore HR-MAS/Micro-Imaging Spectrometer

• Varian 14T (600MHz) NMR System
• Wide bore
• HR-MAS and Micro-imaging capabilities
•Equipped with a custom designed, water cooled, 1000mT/m gradients and high-strength 2nd and 3rd order shims
• Enable
   1) the acquisition of 1H 2D and multinuclear HR-MAS spectra from small samples (6-10mg)
   2) the ability to perform ex vivo and in vivo 3D anatomic, perfusion, diffusion MRI and spectroscopic imaging studies of cultured tissues and cells, and transgenic mice
   3) the ability to utilize novel hyperpolarized 13C labeled substrates and 13C spectroscopic imaging techniques to identify exciting new biomarkers.
• 40mm, single and dual tuned volume RF coils are available for 1H and 13C MRI and MRS

Surbeck Laboratory for Advanced Imaging

The California Institute for Quantitative Biomedical Research (QB3) houses the Surbeck Laboratory for Advanced Imaging which includes a 7T GE 950 whole body scanner, a new 750 3T research scanner, microscopy, computational and other facilities. The only current clinical 13C Dynamic Nuclear Polarizer in the world is installed in a clean room directly adjacent to the 3T scan room to permit human investigations of this new technology. A second, commercial DNP polarizer (Oxford Instrument’s HyperSense) is sited between the 3T and 7T MR scanners to allow development and animal studies on both scanners. A new GE SpinLab multisample polarizer and QC unit prototype (funded by NIH Shared Equipment grant S10RR029570) is also sited between the human MR scanners and is used currently for preclinical animal studies and is being developed for future human HP 13C-pyruvate studies. There is also an electronics shop, a machine shop and a server room that is dedicated to meet the heavy computational needs of the research programs in the building. There is also a three-room suite for animal management and housing. The Surbeck Laboratory also has facilities for managing human subjects including offices, a changing room, preparation room, and restroom.

UCSF Biomedical NMR Laboratory

The Biomedical NMR Lab on the Mission Bay Campus at UCSF occupies 1660 sq. ft. and currently houses a high field multi-nuclear 500 MHz HR-MAS NMR spectrometer and 600 MHz multinuclear micro-imaging spectrometer that are uniquely integrated with two Oxford Instruments DNP polarizers, one HyperSense commercial instrument funded through NIH equipment grant S10RR024587 and a “test-bed” instrument purchased through NIH P41EB013598. A large number of high- field spectrometers (400 MHz, 2 500 MHz, 2 600 MHz and a 800 MHz) with multi-nuclear capabilities and sensitive cryo-probes are also available within the UCSF NMR lab. Both magnets are dedicated to running biomedical samples with complimentary features, including high-resolution magic angle spinning (HR-MAS) spectroscopy, and micro-imaging capabilities that allow for NMR studies of biopsy and surgical tissues, cell and tissue cultures and murine models of cancer. By placing the HyperSenseTM polarizer adjacent to the two high field NMR spectrometers, we can take advantage of the unique features of each system.